Aflatoxin (AFT) is a class of chemically similar compounds, all derivatives of dihydrofuran coumarin. Aflatoxin is a secondary metabolite produced primarily by Aspergillus flavus, a. parasiticus, with the highest incidence of aflatoxin in food and feed in hot and humid regions. They are found in soil, animals and plants, and various nuts. Especially, they are easy to pollute peanuts, corn, rice, soybeans, wheat and other grain and oil products. They are the most toxic and harmful to human health. On October 27, 2017, the World Health Organization’s International Agency for Research on Cancer published a preliminary list of carcinogens, aflatoxins in a list of carcinogens.
Aflatoxins, a group of chemically similar compounds, have been isolated and identified from 12 species including B1, B2, G1, G2, M1, M2, P1, Q, H1, GM, B2a and toxic alcohol. Aspergillus flavus The basic structure of the toxin is difuran ring and coumarin, and B1 is a derivative of dihydrofuran oxaphthalene. It contains a difuran ring and an oxaphthalene (coumarin). The former is basic toxicity. The latter structure is associated with carcinogenesis. M1 is a metabolite derived from aflatoxin B1 by hydroxylation in the body. The main molecular formula of aflatoxin contains B1, B2, G1, G2, M1, M2, etc. Among them, M1 and M2 Mainly in milk. B1 is the most toxic and carcinogenic substance.
Aflatoxin is much more toxic than cyanide, arsenide and organic pesticides, with B1 being the most toxic. When a person consumes a large amount, acute poisoning may occur, and acute hepatitis, hemorrhagic necrosis, hepatic steatosis, and bile duct hyperplasia may occur. When the trace is continuously ingested, it can cause chronic poisoning, growth disorders, and cause fibrous lesions, resulting in fibrous tissue hyperplasia. AFT also ranks first in carcinogenicity and is one of the most known carcinogens.
Aflatoxin-producing bacteria and toxin-producing conditions The most important species capable of producing aflatoxins are Aspergillus flavus and Aspergillus parasiticus, in addition to Aspergillus niger, Aspergillus oryzae, Aspergillus oryzae, Penicillium spp. , expansion of Penicillium, Penicillium digitum, etc., Mucor, Fusarium, Rhizopus, Streptomyces, etc. can also produce aflatoxins. The conditions under which they produce aflatoxin are as follows: substrate, temperature, pH, relative humidity.
Thin film chromatography and liquid chromatography are traditional detection methods. Due to its long detection period and the complexity of the procedures, the shortcomings of the complex reagents are far from meeting the requirements of modern testing. With the continuous development of modern science and technology, especially the immunochemistry of biochemistry, the continuous development of molecular biology has been created. Less rapid, simple and specific, sensitive, low-cost and suitable aflatoxin detection method. The new method represented by gold standard test paper and ELISA kit has been widely used in many countries.GLORY SCIENCE CO.,LTD scientists also take various measures to guarantee antibody quality, for example ,we would do restrict quality examination of each batch of antibody ,guarantee the preserved quantity of the low subcultured hybridoma in the liquid nitrogen,prevent the high subcultured ones from producing antibody, shorten the time of in vitro and animal in vivo culture.