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Detection of aflatoxin

Detection of aflatoxin

Aflatoxin is designated as a class of carcinogens by the World Health Organization (WHO) Cancer Research Institute and is a highly toxic substance. The harm of aflatoxin is the destruction of human and animal liver tissue, it can lead to severe liver cancer and even death. Aflatoxin B1 is more common in natural pollution of food, its toxicity and carcinogenicity is also the strongest.

Aflatoxin B1 is the most dangerous carcinogen, it often be detected in corn, peanuts, cotton seeds and some dried fruits. They can produce fluorescence in the ultraviolet radiation, according to the different color of the fluorescent, it will be divided into B and G class two categories.

When the aflatoxin into the body, the amount is more higher in the liver than other organs, indicating that the liver is the most vulnerable organs to aflatoxin. Aflatoxin can also be detected in Kidney, spleen and adrenal gland, but the muscles generally can not be detected. If humans do not continuously take aflatoxin, it is generally not accumulated in the body. In general, about one week after the intake of aflatoxin, most of it is drained through respiration, urine, and feces.

The federal government has ruled that aflatoxin levels in food and dairy feed should not exceed 15μg/kg. The content of milk should not exceed 0.5 μg/kg, the content of other animal feed shall not exceed 300 μg/kg.

Thin film chromatography and liquid chromatography are the most widely used methods in our country. Because of its long detection cycle, complex procedures and other shortcomings, it already can not meet the requirements of modern testing. With the continuous development of modern science and technology, especially the immunology of biochemistry, molecular biology continue to create a lot of fast, simple and specific, high sensitivity, low cost aflatoxin detection methods. Immunoassay, which is designed using a highly specific monoclonal antibody or polyclonal antibody,  is also the most commonly used method for aflatoxin detection. Immunoassay usually involves radioimmunoassay (RIA) and Enzyme-linked of Immunosorbent Assay (ELISA), both of which can quantitatively determine the amount of aflatoxin. Total aflatoxin ELISA kit (GLORY SCIENCE CO., LTD, Product No.:AE004) is usually used.

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