Description

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Host species/Isotype:Rabbit/IgG

Cross reaction: Belt Jellyfish.

Immunogen: KLH conjugated synthetic peptide derived from belt jellyfish Green Fluorecent protein :25-120/238 .

Storage instructions:Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4°C.

Concentration:1mg/ml

Specification:100ul

Purity:affinity purified by Protein A

Clonality:Polyclonal

Molecular weight:27kDa

Tested applications: 

WB=1:500-2000

ELISA=1:500-1000

IHC-P=1:400-800

IHC-F=1:400-800

IF=1:100-500