The kit uses a double-antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with Giardia antibodies, the specimens, HRP-labeled detection antibodies were sequentially added, incubated and thoroughly washed. Using the substrate TMB to develop color, TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The absorbance (OD value) was measured at a wavelength of 450 nm using a microplate reader, and compared with the CUT OFF value to determine the presence or absence of Giardia lamblia in the specimen.
Method type : Sandwich ELISA
Application : ELISA
Research Area : Immunology->Innate Immunity->Complement->Alternative Pathway, Immunology->Innate Immunity->Complement->Other
Sample Type :Feces
Plate : Pre-coated,Strips (12 x 8)
Restrictions : For Research Use only
Storage : 2 °C – 8 °C
Storage Comment : Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date : 12 months
Size : 96T
Availability : 3-5 working days
Precision : Intra-assay Precision (Precision within an assay) CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays) CV%<15% Three samples of known concentration were tested in twenty assays to assess.
- Test validity: the average of Positive control well≥1.00; the average of Negative control well ≤0.15.
- Calculate Critical (CUT OFF): Critical= the average of Negative control well + 0.15.
Negative Result: sample OD< Calculate Critical (CUT OFF) is Negative.
Positive Result: sample OD≥ Calculate Critical (CUT OFF) is Positive.