$0.00
2.9%
Description
Principle of test
The kit is for the quantitative level of TMAO in the sample, adopt purified Human TMAO to coat microtiter plate, make solid-phase antibody, then add TMAO to wells, Combine TMAO antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of TMAO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For the quantitative determination of Human TMAO acid concentrations.
Detection of species:Human.
Detection medium: serum, plasma, tissue homogenates, cell culture supernates .
| Reagent | Quantity |
| Microelisa Stripplate | 12well×8strips |
| Standard | 6×0.3ml |
| HRP-Conjugate Reagent | 1×10ml |
| Sample Diluent | 1×6ml |
| Chromogen Solution A | 1×6ml |
| Chromogen Solution B | 1×6ml |
| Stop Solution | 1×6ml |
| 20X Wash Solution | 1×25ml |
| Sealed bags | 1 |
| User manual | 1 |
| Closure plate membrane | 2 |
Note: Standard(S0→S5) concentration was followed by:0, 10, 20, 40, 80, 160 μg/ml
STORAGE CONDITIONS ： The unopened kit shall be stored at [2-8 ℃] .
For opened kit can be stored at [2-8 ℃] for up to 1 month. If not be used recently.
1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated
by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis
versus the corresponding concentration on the horizontal (X) axis.
2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean
value of the zero standard before result interpretation. Construct the standard curve using graph paper or
statistical software.
3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal
line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding
concentration.
4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can
cause variation in result. Each user should obtain their own standard curve.
5. The sensitivity by this assay is 1.0μg
6. Standard curve
DESCRIPTION ：
1. The standard curve is drawn under ideal conditions, and is just for reference rather than the actual standard
curve diagram of the kit.
2. It is advisable to establish proper assay data and standard curve according to respective laboratory
conditions.
https://www.glorybios.com/product/28203/
