Growth-differentiation factor 15 (GDF15), also known as MIC-1, is a secreted member of the transforming growth factor (TGF)-β superfamily, as a novel antihypertrophic regulatory factor in the heart. GDF-15 / GDF15 is not expressed in the normal adult heart but is induced in response to conditions that promote hypertrophy and dilated cardiomyopathy and it is expressed highly in liver. GDF-15 / GDF15 has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF-15 / GDF15 is synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein. GDF-15 / GDF15 overexpression arising from an expanded erythroid compartment contributes to iron overload in thalassemia syndromes by inhibiting hepcidin expression.This GDF15 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human GDF15 . It contains Human GDF15 capture antibody, Human GDF15 detector antibody (HRP) and a highly purified recombinant Human GDF15 protein. This Pair Set is at affordable price for researchers.
Detection principle:Solid Phase Sandwich ELISA.
Specificity:Quantitative determination of Human GDF15.
ELISA antibody pair kit (non-ready) components:
1. Capture Antibody 1 mg/mL of mouse anti-Human GDF15 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human GDF15 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.15 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 15 ng of recombinant Human GDF15. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 300 pg/mL is recommended.
Mode of transport:This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
1.Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
2.Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
3.Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.