The kit uses a double antibody sandwich assay to determine the level of human hypoglycosylated IgA1 (UdgIgA1) in the specimen. The microplate was coated with a purified human hypoglycosylated IgA1 (UdgIgA1) capture antibody to prepare a solid phase antibody, and human hypoglycosylated IgA1 (UdgIgA1) was sequentially added to the coated microwell, followed by HRP-labeled detection antibody. The antibody-antigen-enzyme-labeled antibody complex is formed by binding, and after thorough washing, the substrate TMB is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with human hypoglycosylated IgA1 (UdgIgA1) in the sample. The absorbance (OD value) was measured by a microplate reader at a wavelength of 450 nm, and the content of human hypoglycosylated IgA1 (UdgIgA1) in the sample was calculated by a standard curve.
Method type : Sandwich ELISA
Minimum Detection Limit :0.1 ng/mL
Detection Range :3.125 ng/mL – 100 ng/mL
Application : ELISA
Purpose : For the quantitative determination of target substances concentrations.
Research Area : Immunology->Innate Immunity->Complement->Alternative Pathway, Immunology->Innate Immunity->Complement->Other
Sample Type : serum, plasma, Urine, tissue samples, cell culture supernates
Plate : Pre-coated,Strips (12 x 8)
Restrictions : For Research Use only
Storage : 2 °C – 8 °C
Storage Comment : Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date : 12 months
Size : 96T
Availability : 3-5 working days
Precision : Intra-assay Precision (Precision within an assay) CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays) CV%<15% Three samples of known concentration were tested in twenty assays to assess.
Linearity : To assess the linearity of the assay, samples were spiked with hig3h concentrations of rat ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Typical Data : These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 1000 0.088 0.090 0.089 500 0.135 0.142 0.139 250 0.227 0.237 0.232 125 0.324 0.341 0.333 62.5 0.583 0.598 0.591 31.25 0.847 0.864 0.856 15.62 1.228 1.235 1.232 0 2.155 2.199 2.177