Reactivity : Human
Method Type : Sandwich ELISA
Minimum Detection : 1800pg/ml
Detection Range : 20 pg/ml -1200 pg/ml
Application : ELISA
Purpose : For the quantitative determination of Human (UBN1) concentrations.
Research Area : Tags & Cell Markers Subcellular Markers Plasma Membrane Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins Signal Transduction Signaling Pathway Calcium Signaling Calcium Binding Proteins Cancer Invasion/microenvironment ECM Cell adhesion Cadherins
Sample Type : serum, plasma, Urine, tissue samples, cell culture supernates
Components : Reagent Quantity
Microelisa Stripplate 12well?8strips
Standard: 90?mol/L 1?0.5ml
Standard Diluent 1?1.5ml
HRP-Conjugate Reagent 1?6ml
Sample Diluent 1?6ml
Chromogen Solution A 1?6ml
Chromogen Solution B 1?6ml
Stop Solution 1?6ml
Wash Solution 1?20ml?30 fold
User manual 1
Adhesive Strip 36
Precoated strips : Pre-coated,Strips (12 x 8)
Restrictions : For Research Use only
Storage : -20 øC,4 øC
Storage Comment : Store at 4øC for 6 months, at -20øC for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date : 12 months
Handling Advice : The kit takes out from the refrigeration should be balanced 15-30 minutes in the room temperature, if the coated ELISA plates have not been used up after opening, the plate should be stored in sealed bag.
Washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilution. Washing does not affect the result.
Pipette sample with pipettors each step, and proofread its accuracy frequently, avoids the experimental error. Pipette sample within 5 min, if the number of sample is much, recommend using multichannel pipettor.
If the testing material content is excessively high (The sample OD is higher than the first standard well)?please dilute sample (n-fold).
Adhesive Strip only limits the disposable use to avoid cross-contamination.
The substrate should be preserved evade the light.
Please refer to use instruction strictly. The test result determination must take the microtiter plate reader as a standard.
All samples, washing buffer and each kind of reject should refer to infective material process.
Do not mix reagents with those from other lots.