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Human Oxytocin (OT)ELISA Kit

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Human Oxytocin (OT)ELISA Kit

$586.00 $492.00

16724. In stock N/A .

Description

The kit is for the quantitative level of OT in the sample, adopt purified Human OT to coat microtiter plate, make solid-phase antibody, then add OT to  wells, Combine OT antibody with  labeled HRP  to form antibody-antigen-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of  OT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reactivity :Human

Method type : Sandwich ELISA

Minimum Detection Limit :1pg/ml

Detection Range :1.2pg/ml-100 pg/ml

Application : ELISA

Purpose : For the quantitative determination of target substances concentrations.

Research Area : Immunology->Innate Immunity->Complement->Alternative Pathway, Immunology->Innate Immunity->Complement->Other

Sample Type : serum, plasma, Urine, tissue samples, cell culture supernates

Plate : Pre-coated,Strips (12 x 8)

Restrictions : For Research Use only

Storage : 2 °C - 8 °C

Storage Comment : Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date : 12 months

Size : 96T

Availability : 3-5 working days

Precision : Intra-assay Precision (Precision within an assay) CV%<15%   Three samples of known concentration were tested twenty times on one plate to assess.   Inter-assay Precision (Precision between assays) CV%<15%   Three samples of known concentration were tested in twenty assays to assess.

Linearity : To assess the linearity of the assay, samples were spiked with hig3h concentrations of rat ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.