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Human Lep-IgG ELISA Kit

Human Lep-IgG ELISA Kit

$524.00

Reagent Quantity Microelisa Stripplate 12well×8strips Standard: 90?mol/L 1×0.5ml Standard Diluent 1×1.5ml HRP-Conjugate Reagent 1×6ml Sample Diluent 1×6ml Chromogen Solution A 1×6ml Chromogen Solution B 1×6ml Stop Solution 1×6ml Wash Solution 1×20ml×30 fold User manual 1 Adhesive Strip 105

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E6272. In stock N/A , .

Description

Principle of the assay
The kit assay Lep-IgG level in the sample,use Purified Lep-IgG antibody to coat microtiter plate wells, make solid-phase antigen, then add Lep-IgG to wells, Combined With Lep-IgG antibody, after washing and removing non-combinative antibody and other components ,then Combined Lep-IgG antibody which with HRP labeled become antibody–antigen – enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge Lep-IgG exist in the sample or not.