Description

Principle of the assay
The kit assay Lep-IgG level in the sample，use Purified Lep-IgG antibody to coat microtiter plate wells, make solid-phase antigen, then add Lep-IgG to wells, Combined With Lep-IgG antibody, after washing and removing non-combinative antibody and other components ,then Combined Lep-IgG antibody which with HRP labeled become antibody–antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge Lep-IgG exist in the sample or not.