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Human Adenovirus (ADV) ELISA Kit

Human Adenovirus (ADV) ELISA Kit

$328.00

Principle of the assay
The kit assay ADV level in the sample,use Purified ADV antibody to coat microtiter plate wells, make solid-phase antibody, then add ADV to wells, Combined With ADV, after washing and removing non-combinative antigen and other components ,then Combined ADV antibody which with HRP labeled become antibody – antigen – enzyme-antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge ADV exist in the sample or not.

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Description

Principle of the assay
The kit assay ADV level in the sample,use Purified ADV antibody to coat microtiter plate wells, make solid-phase antibody, then add ADV to wells, Combined With ADV, after washing and removing non-combinative antigen and other components ,then Combined ADV antibody which with HRP labeled become antibody – antigen – enzyme-antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge ADV exist in the sample or not.