The application of monoclonal antibody in animal food safety detection

For the past few years, the food in our country has a great security risk, and the government attaches great importance to the safety control of food chain from farm to table. Therefore, detection and monitoring of pollutants and hazards in food are increasingly important, which requires fast, accurate and sensitive detection techniques. In recent years, with the development of monoclonal antibody technology and the commercialization of immunization kits, people are increasingly inclined to establish food detection systems based on monoclonal antibodies (GLORY SCIENCE CO., LTD).

The problem of veterinary drug residue in animal food is attracting more and more attention from the international community.In the world, especially in the developed west, they have stepped up their testing of veterinary residues in animal foods in international trade.

1. Beta stimulants

Clenbuterol hydrochloride, lake toba, salbutamol can promote feed conversion rate, increase the lean production, as a kind of illegal feed additive was used by many professional raise animals and feed processing enterprises. However, beta stimulants can cause heart palpitations, headaches, dazzliness, nausea and even kidney damage, Many countries in the world, including China, have banned such drugs. The preparation technology of clentro hydrochloride testing kit is becoming more and more mature. There are already a lot of finished products in the market. Sawaya obtained a monoclonal antibody for albuterol using the technique of hybridioma, and established a rapid detection method for the drug residues of albuterol in food.

2. Antibiotics

Fluoroquinolone drugs, chloramphenicol, etc. Which are the important  drugs used in anti-infection treatment of livestock and poultry. The residual effects on human health are becoming more and more important. China has classified it as the key monitoring project of veterinary drug residue in animal food and has set strict residue limit standards. The monoclonal antibody of tetracycline and chloramphenicol monoclonal antibody have been widely used in food detection, Loomans have used chloramphenicol ELISA (GLORY SCIENCE CO.,LTD,Product No.: AE013) kit to detected chloramphenicol in eggs.

3. The hormonal

The estradiol and hexanestrol, as a growth enhancer, it is widely used in animal husbandry, but these hormones can destroy endocrine and potentially be carcinogenic. The artificial immunogen was prepared by using mixed acid anhydride method for the derivation of semi – antigen by Yellow, and a monoclonal cell line of monoclonal antibody with stable secretion was selected, which laid the foundation for the establishment of the immunoassay technique.

Monoclonal antibody technology is a new technology of rapid development in recent years, with strong specificity, high sensitivity, convenient and quick, analysis of large capacity, low testing cost, and the advantages of safe and reliable, has become the 21st century the most competitive and challenging test analysis of new technology.