Description

The rotavirus (RV) antigen rapid detection card uses colloidal gold immunochromatography for rapid detection of group A rotavirus in Canine. After the sample taken from the stool was added to the well, it was moved along the chromatographic membrane together with the colloidal gold-labeled anti-RV monoclonal antibody. If the RV antigen is present in the sample, it binds to the antibody on the test line to display wine red. If the RV antigen is not present in the sample, no color reaction occurs.
Assay Procedure:
1. Use a cotton swab to collect freshly excreted stool samples or sample directly from the rectum. If you are screening for rotavirus in animals without diarrhea, use rectal sampling. It is not recommended to collect dry stool samples because the collection of dry feces often results in false positives due to excessive sample collection.
2. Immediately insert the cotton swab into the collection tube containing the sample buffer, rotate the cotton swab repeatedly on the tube wall at least 10 times, and mix the solution to dissolve the sample as much as possible in the solution. Squeeze the cotton swab on the tube wall to allow the liquid to be squeezed out as much as possible and discard the cotton swab.
3. If the stool particles are large, it can be allowed to stand for 1-2 minutes to precipitate large particles.
4. Remove the test card from the sealed bag. Place the test card on a flat surface, use a pipette to draw the upper clear liquid from the collection tube, and slowly and drop 5 drops to the well with the“S”mark.
5. Leave at room temperature for 10-15 minutes to judge the result. The result of more than 15 minutes is invalid.
Result Analysis:
1. Positive: In the observation hole, the detection line region (T) and the control line region (C) simultaneously appear a purple-red line
2. Negative: Within the observation well, only a reddish line appears in the control line (C).
3. Failure: In the observation hole, no color line appears in the control line area (C) and the detection line area (T); or only the detection line area (T) appears color line.
Specification: 20T
Valid Period: The kit is stored at 2～30°C, the validity period is 24 months.